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MB Sample ID: SA163298
Local Sample ID: | LPS13C3 |
Subject ID: | SU001821 |
Subject Type: | Cultured cells |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
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Treatment:
Treatment ID: | TR001834 |
Treatment Summary: | high-glucose DMEM (Gibco) containing 10% FBS and 1% penicillin/streptomycin (Gibco). Isotopic labeling was achieved through 30 min treatments with media containing 4.5 g/L U-13C-glucose (100% of the total glucose content; Cambridge Isotopes); parallel cultures were treated with 4.5 g/L natural-abundance glucose. Simultaneous to the introduction of the labeled substrate, subsets of both unlabeled and labeled cultures were treated with LPS (added to culture media to a final concentration of 1 μg/mL) for the duration of the labeling protocol. |