Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA163299

Local Sample ID:	LPS13C1
Subject ID:	SU001821
Subject Type:	Cultured cells
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116

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Treatment:

Treatment ID:	TR001834
Treatment Summary:	high-glucose DMEM (Gibco) containing 10% FBS and 1% penicillin/streptomycin (Gibco). Isotopic labeling was achieved through 30 min treatments with media containing 4.5 g/L U-13C-glucose (100% of the total glucose content; Cambridge Isotopes); parallel cultures were treated with 4.5 g/L natural-abundance glucose. Simultaneous to the introduction of the labeled substrate, subsets of both unlabeled and labeled cultures were treated with LPS (added to culture media to a final concentration of 1 μg/mL) for the duration of the labeling protocol.

Treatment ID:

TR001834

Treatment Summary:

high-glucose DMEM (Gibco) containing 10% FBS and 1% penicillin/streptomycin (Gibco). Isotopic labeling was achieved through 30 min treatments with media containing 4.5 g/L U-13C-glucose (100% of the total glucose content; Cambridge Isotopes); parallel cultures were treated with 4.5 g/L natural-abundance glucose. Simultaneous to the introduction of the labeled substrate, subsets of both unlabeled and labeled cultures were treated with LPS (added to culture media to a final concentration of 1 μg/mL) for the duration of the labeling protocol.