Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA169046

Local Sample ID:	FIP200_KO_EBSS1
Subject ID:	SU001895
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Not applicable

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Treatment:

Treatment ID:	TR001908
Treatment Summary:	MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. For autophagy induction, 2 × 105 cells were seeded on 6-well plates and the next day, cells were washed twice with PBS and incubated in amino acid-free EBSS (starvation medium; Thermo Fisher Scientific 24010-043) in the presence or absence of bafilomycin A1 (Selleck Chemicals) for 30 min at 37 ºC.

Treatment ID:

TR001908

Treatment Summary:

MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. For autophagy induction, 2 × 105 cells were seeded on 6-well plates and the next day, cells were washed twice with PBS and incubated in amino acid-free EBSS (starvation medium; Thermo Fisher Scientific 24010-043) in the presence or absence of bafilomycin A1 (Selleck Chemicals) for 30 min at 37 ºC.