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MB Sample ID: SA173466
Local Sample ID: | Corynebacterium striatum St.29-1 (50uM_LCA_pH7) |
Subject ID: | SU001929 |
Subject Type: | Bacteria |
Subject Species: | Bacteria |
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Treatment:
Treatment ID: | TR001941 |
Treatment Summary: | Under anaerobic conditions, isolated bacteria strains were cultured together with 50 μM of CDCA, 3-oxo-Δ4-LCA, or LCA to screen for their bile acid metabolism in a 96-deep well plate covered with a gas-permeable membrane. 20 μL of bacterial culture in exponential to stationary phase was inoculated into 1 mL of Wilkins-Chalgren Anaerobe (WCA) media adjusted to pH 7 (using MOPS buffer solution) or pH 9 (TAPS buffer solution). |