Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA173889

Local Sample ID:	Parabacteroides merdae St.3-2 (50uM_3-oxo-Δ4-LCA_pH7)
Subject ID:	SU001929
Subject Type:	Bacteria
Subject Species:	Bacteria

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Treatment:

Treatment ID:	TR001941
Treatment Summary:	Under anaerobic conditions, isolated bacteria strains were cultured together with 50 μM of CDCA, 3-oxo-Δ4-LCA, or LCA to screen for their bile acid metabolism in a 96-deep well plate covered with a gas-permeable membrane. 20 μL of bacterial culture in exponential to stationary phase was inoculated into 1 mL of Wilkins-Chalgren Anaerobe (WCA) media adjusted to pH 7 (using MOPS buffer solution) or pH 9 (TAPS buffer solution).

Treatment ID:

TR001941

Treatment Summary:

Under anaerobic conditions, isolated bacteria strains were cultured together with 50 μM of CDCA, 3-oxo-Δ4-LCA, or LCA to screen for their bile acid metabolism in a 96-deep well plate covered with a gas-permeable membrane. 20 μL of bacterial culture in exponential to stationary phase was inoculated into 1 mL of Wilkins-Chalgren Anaerobe (WCA) media adjusted to pH 7 (using MOPS buffer solution) or pH 9 (TAPS buffer solution).