Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA174641

Local Sample ID:	1401_L
Subject ID:	SU001945
Subject Type:	Mammal
Subject Species:	Ovis aries
Taxonomy ID:	9940
Animal Inclusion Criteria:	Pregnant

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Treatment:

Treatment ID:	TR001957
Treatment Summary:	Animals in the CORT and CORT+DCA groups were implanted with pellets of cortisol hemisuccinate (Innovative Research, Sarasota, FL) or infused with cortisol hemisuccinate (Solu-Cortef, Pfizer, Inc) using an ambulatory pump (3D Micro Infusion Pump; Strategic Applications Inc.; Lake Villa, IL) to provide dosing of cortisol at 1mg/kg/day beginning on day 115-116 of gestation until the end of the experiment when the animals were sacrificed. This dose of cortisol increased maternal cortisol concentrations to approximately 1.5-fold, mimicking the rise in cortisol in moderate stress. To produce a regular pattern of labor, ewes were infused with oxytocin (Aspen Veterinary Resources) beginning at day 138-140 of gestation; oxytocin was infused over 5 minutes every 30 minutes (820 µU/kg/min iv;) using an infusion pump controlled by a timed controller ChronTrol Corp, Inc). DCA infusion was started 48 hours after the start of oxytocin. DCA was administered as an intravenous bolus of 25 mg/kg over 3 minutes, followed by an infusion of 12.5 mg DCA/kg/hour for 8 hours, and an infusion of 6.25 mg DCA/kg/h for the next 8 hours. These doses are similar to those that significantly decrease circulating lactate concentrations in humans. The timed infusions of oxytocin continued for 72 hours, and the study was terminated after 24 hours of DCA and/or 72 hours of oxytocin infusion, unless ewes were in the process of delivery or birth occurred.

Treatment ID:

TR001957

Treatment Summary:

Animals in the CORT and CORT+DCA groups were implanted with pellets of cortisol hemisuccinate (Innovative Research, Sarasota, FL) or infused with cortisol hemisuccinate (Solu-Cortef, Pfizer, Inc) using an ambulatory pump (3D Micro Infusion Pump; Strategic Applications Inc.; Lake Villa, IL) to provide dosing of cortisol at 1mg/kg/day beginning on day 115-116 of gestation until the end of the experiment when the animals were sacrificed. This dose of cortisol increased maternal cortisol concentrations to approximately 1.5-fold, mimicking the rise in cortisol in moderate stress. To produce a regular pattern of labor, ewes were infused with oxytocin (Aspen Veterinary Resources) beginning at day 138-140 of gestation; oxytocin was infused over 5 minutes every 30 minutes (820 µU/kg/min iv;) using an infusion pump controlled by a timed controller ChronTrol Corp, Inc). DCA infusion was started 48 hours after the start of oxytocin. DCA was administered as an intravenous bolus of 25 mg/kg over 3 minutes, followed by an infusion of 12.5 mg DCA/kg/hour for 8 hours, and an infusion of 6.25 mg DCA/kg/h for the next 8 hours. These doses are similar to those that significantly decrease circulating lactate concentrations in humans. The timed infusions of oxytocin continued for 72 hours, and the study was terminated after 24 hours of DCA and/or 72 hours of oxytocin infusion, unless ewes were in the process of delivery or birth occurred.