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MB Sample ID: SA174771
Local Sample ID: | ST9 |
Subject ID: | SU001949 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 mice (Wild-type) |
Age Or Age Range: | six weeks old |
Gender: | Female |
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Treatment:
Treatment ID: | TR001961 |
Treatment Summary: | Six-week-old female wildtype (WT) C57BL/6 mice acquired from Jackson Laboratories (Bar Harbor, ME) were housed in an AAALAC-accredited specific-pathogen-free animal facility located at the grounds of the University of South Carolina School of Medicine for the entirety of all experiments. Mice within the facility were housed within polycarbonate cages containing cellulose fiber chips as bedding in a temperature and humidity-controlled environment. After a 2-week acclimatization period, the mice were divided randomly into two groups that would be administered a single 100µl intraperitoneal injection containing sterile corn oil (VEH group) or an intraperitoneal injection of 10µg/kg TCDD suspended within sterile corn oil (TCDD group). At the 72h time point following TCDD or VEH exposure, the mice were humanely euthanized by an overdose of inhaled isoflurane. Cecal content and blood sera were collected at the time of necropsy. |