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MB Sample ID: SA176009
| Local Sample ID: | Acatenella 5 |
| Subject ID: | SU001973 |
| Subject Type: | Other organism |
| Subject Species: | Alexandrium catenella; Alexandrium tamarense |
| Taxonomy ID: | 2925; 2926 |
| Genotype Strain: | CCMP 1719; CCMP 2023 |
| Gender: | Not applicable |
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Treatment:
| Treatment ID: | TR001985 |
| Treatment Summary: | Metabolomes of toxic versus non-toxic species were compared using the following experimental pairings: A. tamarense (n=15) with A. catenella (n=15) (Experiment 1) and A. tamarense (n=15) with A. pacificum (n=15) (Experiment 2). The same non-toxic strain of A. tamarense was used in both experiments but the two experiments were conducted separately, in different months, to make the experiment manageable based on availability of batches grown from stock cultures. For both experiments, Alexandrium spp. cultures at a cell density of 12,000 to 13,000 cells mL-1 were split into fifteen 300 mL subcultures of each species which grew for two days. At the end of each experiment, during harvesting, a 1.0 mL aliquot from each culture was preserved with Lugol’s solution to measure cell concentrations |
| Treatment Protocol Filename: | Differentiating_toxic_and_nontoxic_congeneric_harmful_algae_using_the_non-polar_metabolome_protocol.pdf |
| Treatment: | Species comparison |
