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MB Sample ID: SA183469
| Local Sample ID: | 51 |
| Subject ID: | SU002022 |
| Subject Type: | Other organism |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
| Genotype Strain: | CCMP1335 |
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Treatment:
| Treatment ID: | TR002034 |
| Treatment Summary: | The diatom was grown in organic-carbon free L1 medium (Guillard and Hargraves 1993) prepared in acid washed glass containers at a salinity of 35 (Harrison et al. 1980) for one week prior to the start of the experiment. Cultures were grown with a 16:8 h light:dark cycle under 160 µmol photons m-2 s-1 at 18°C and checked for bacterial contamination by plating on rich medium (½ YTSS). On Day 0 of the experiment, diatoms were transferred into 1.9 L culture flasks containing 1000 ml of medium made with 13C-bicarbonate (final concentration of ~ 2x103 cells ml-1). One flask was kept as an L1 medium control without organisms. The three strains of heterotrophic bacteria were grown overnight in rich medium made with artificial seawater, either ½ YTSS medium (R. pomeroyi and Stenotrophomonas) or 1/10 YTSS medium (P. dokdonensis). Cells were harvested in exponential growth phase and washed five times in the same artificial sea water used for preparing the L1 medium. The bacteria were inoculated in equal proportions of OD600 into 15 flasks containing diatoms, with a final combined concentration of ca. 1x105 cells ml-1. One set of three flasks remained axenic. Three co-culture flasks were sacrificed at 4 pm days 3 and 15, and the axenic flasks day 15. |
| Treatment Protocol Filename: | 3_Treatment protocol_UGA_phytoplankton_ Aug2021.docx |
| Treatment Protocol Comments: | Details are in 3_Treatment protocol_UGA_phytoplankton_ Aug2021.docx. |
| Treatment: | Incubation of a diatom strain with and without bacterial strains |
