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MB Sample ID: SA184775
| Local Sample ID: | 71-9_35b |
| Subject ID: | SU002042 |
| Subject Type: | Plant |
| Subject Species: | Populus tremula x Populus alba |
| Taxonomy ID: | 80863 |
| Genotype Strain: | INRA-717-1B4 |
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Treatment:
| Treatment ID: | TR002054 |
| Treatment Summary: | Leaf tissue from Populus tremula x Populus alba was transformed using CRISPR-Cas9 to create functional knockouts of the UGT71L1 gene. Positive transformants were selected with Kanamycin and sequencing was conducted to confirm transformation. Clones of either wild type plants, knockouts (frame shift mutations, both alleles), intermediates (Frameshift mutation, one allele), or empty vectors (no gRNA for Cas9 construct). Plants were then grown under identical conditions for three months in a greenhouse. |
| Treatment: | Genetic |
| Plant Growth Location: | Greenhouse |
| Plant Watering Regime: | As required |
