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MB Sample ID: SA192446

Local Sample ID:	exp9_hilic_neg_02
Subject ID:	SU002128
Subject Type:	Invertebrate
Subject Species:	Caenorhabditis elegans
Taxonomy ID:	6239

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Treatment:

Treatment ID:	TR002140
Treatment Summary:	The compared groups are different extraction protocols using a Taguchi Design of Experiments (DOE) approach. 3 different types of: solvent, volume, extraction time, and reconstitution solvent were investigated. Solvents included: IPA, MeOH, and 1:1 1-Butanol:MeOH for non-polar extractions followed by an 80:20 MeOH/H2O sequential polar extraction. Volumes of 0.5, 1.0, and 3.0 mL were used; Times: 0.5, 2.0, or 12.0 hours, and reconstitution solvents: IPA, 60/40 H2O/ACN, or 50/50 IPA/ACN (non-polar), and H2O, MeOH, or 80/20 MeOH/H2O (polar). Detailed information on the 9 different experimental conditions can be found in the attached manuscript or methods.

Treatment ID:

TR002140

Treatment Summary:

The compared groups are different extraction protocols using a Taguchi Design of Experiments (DOE) approach. 3 different types of: solvent, volume, extraction time, and reconstitution solvent were investigated. Solvents included: IPA, MeOH, and 1:1 1-Butanol:MeOH for non-polar extractions followed by an 80:20 MeOH/H2O sequential polar extraction. Volumes of 0.5, 1.0, and 3.0 mL were used; Times: 0.5, 2.0, or 12.0 hours, and reconstitution solvents: IPA, 60/40 H2O/ACN, or 50/50 IPA/ACN (non-polar), and H2O, MeOH, or 80/20 MeOH/H2O (polar). Detailed information on the 9 different experimental conditions can be found in the attached manuscript or methods.