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MB Sample ID: SA203059
Local Sample ID: | m07a |
Subject ID: | SU002202 |
Subject Type: | Bacteria |
Subject Species: | Bacteroides xylanisolvens |
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Treatment:
Treatment ID: | TR002214 |
Treatment Summary: | Germ-free C57BL/6J mice were reared in plastic flexible film gnotobiotic isolators (Class Biologically Clean) at 23 °C under a strict 12-hour light cycle (lights on at 0600h, off at 1800h). Animals were weaned onto an autoclaved, low-fat, plant polysaccharide-rich chow (catalog number 2018S, Envigo) that was administered ad libitum. Five days prior to colonization, 8-week-old male mice were switched to the NAD precursor-sufficient diet; 2 days later, they were either placed on a NAD precursor-deficient diet, or a NA-supplemented diet or were continued on the NAD precursor-sufficient diet. |