Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA205757

Local Sample ID:	OI1_D12_S3_Medium
Subject ID:	SU002228
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment:

Treatment ID:	TR002240
Treatment Summary:	hAMSCs were detached, at passage 7, from the flasks by trypsinization, counted in a Neubauer chamber and seeded at a density of 0.5 × 10 6 cells/flask. Cells were maintained under basal conditions until reaching ~100% confluence, then the basal culture medium was exchanged and supplemented with osteoinductive factors, specifically 10 mM β-glycerophosphate (β-GP, Sigma-Aldrich G9422), 50 µg/mL L-ascorbic acid (Sigma A0278), and 10 nM Dexa (ACROS Organics™ 230300010).

Treatment ID:

TR002240

Treatment Summary:

hAMSCs were detached, at passage 7, from the flasks by trypsinization, counted in a Neubauer chamber and seeded at a density of 0.5 × 10 6 cells/flask. Cells were maintained under basal conditions until reaching ~100% confluence, then the basal culture medium was exchanged and supplemented with osteoinductive factors, specifically 10 mM β-glycerophosphate (β-GP, Sigma-Aldrich G9422), 50 µg/mL L-ascorbic acid (Sigma A0278), and 10 nM Dexa (ACROS Organics™ 230300010).