Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA205780

Local Sample ID:	shchrebp HG-2
Subject ID:	SU002230
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR002242
Treatment Summary:	An engineered miR-30 based ChREBP knockdown (KD) construct was assembled by PCR using previously validated ChREBP shRNA clone,34 followed by an optimized miR-E backbone. Stable podocyte cell line with ChREBP KD were generated by transfection into constructs together with PiggyBac transposase. After selection with 0.5 μg/ml of blasticidin alone or in combination with 1μg/ml puromycin, GFP-positive cells were sorted by FACS, and the top 30% population were collected.

Treatment ID:

TR002242

Treatment Summary:

An engineered miR-30 based ChREBP knockdown (KD) construct was assembled by PCR using previously validated ChREBP shRNA clone,34 followed by an optimized miR-E backbone. Stable podocyte cell line with ChREBP KD were generated by transfection into constructs together with PiggyBac transposase. After selection with 0.5 μg/ml of blasticidin alone or in combination with 1μg/ml puromycin, GFP-positive cells were sorted by FACS, and the top 30% population were collected.