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MB Sample ID: SA210704
| Local Sample ID: | T7.40.LWS2_neg |
| Subject ID: | SU002284 |
| Subject Type: | Plant |
| Subject Species: | Pinus pinaster |
| Taxonomy ID: | 71647 |
| Age Or Age Range: | one-two years |
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Treatment:
| Treatment ID: | TR002296 |
| Treatment Summary: | The experimental layout was based on a factorial design with two factors: temperature and water availability. Before starting the experiment, plants were divided into two chambers for testing two temperatures (30 ºC and 40 ºC), which in turn were split again into two levels of water availability (“low-water-stress”, LWS, and “high-water-stress”, HWS). Consequently, four stress levels, 40 ºC-HWS, 40 ºC-LWS, 30 ºC-HWS, and 30 ºC-LWS were tested. In both chambers, twelve plants were divided into six pools of two plants (three for HWS and three for LWS). These pools of two plants were kept across the sampling and formed the three independent biological replicates analyzed for each stress level. |
| Treatment: | Heat and Drought |
| Treatment Dose: | High Water Stress and Low Water Stress in 30ºC and 40ºC. |
| Treatment Vehicle: | Fitoclima 1200, Aralab Ltd, Sintra, Portugal |
| Plant Growth Support: | Fitoclima 1200, Aralab Ltd, Sintra, Portugal |
| Plant Growth Location: | Oviedo, Asturias |
| Plant Plot Design: | Randomized design |
| Plant Light Period: | During this month, the plants in the growth chamber (Fitoclima 1200, Aralab Ltd, Sintra, Portugal) were kept at a light intensity of 400 µmol m−2·s−1 under long-day conditions (16 h light/8 h dark for photoperiod). |
| Plant Humidity: | Relative humidity (RH) were set to 25 ºC and 50 % RH during the day, and 15 ºC and 60 % RH during the night |
| Plant Temp: | 25ºC, 30ºC, and 40ºC |
| Plant Watering Regime: | Plants were well-watered to field capacity until soil dropped every two days. |
| Plant Nutritional Regime: | efore trial, seedlings had been acclimated over one month inside the chamber and were watered to field capacity with nutritive solution (N:P:K; 5:8:10). |
| Plant Growth Stage: | Two-year-old seedlings |
| Plant Metab Quench Method: | Liquid N2 |
| Plant Harvest Method: | Liquid N2 |
| Plant Storage: | Lyophilized |
