Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA231952

Local Sample ID:	VT_181119_M338_091
Subject ID:	SU002419
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment:

Treatment ID:	TR002431
Treatment Summary:	Plasma samples in 50μL aliquot were mixed with acetonitrile containing 14 stable isotope internal standards at a 2:1 ratio to precipitate proteins. Samples were then equilibrated on ice for 30 minutes and centrifuged for 10 minutes at 13,400 rpm at 4°C. The supernatant was transferred to autosampler vials and kept in a refrigerated autosampler until analysis. Each extract was analyzed in triplicate using a dual column chromatography scheme that includes hydrophilic interaction liquid chromatography (HILIC; XBridge BEH Amide XP HILIC column; Waters, Waltham, MA, 50x2.1mm, 2.5μm) and reversed phase liquid chromatography (RPLC; C18 column; Higgins Analytical, Mountain View, CA, 50x2.1mm, 2.6 μm).

Treatment ID:

TR002431

Treatment Summary:

Plasma samples in 50μL aliquot were mixed with acetonitrile containing 14 stable isotope internal standards at a 2:1 ratio to precipitate proteins. Samples were then equilibrated on ice for 30 minutes and centrifuged for 10 minutes at 13,400 rpm at 4°C. The supernatant was transferred to autosampler vials and kept in a refrigerated autosampler until analysis. Each extract was analyzed in triplicate using a dual column chromatography scheme that includes hydrophilic interaction liquid chromatography (HILIC; XBridge BEH Amide XP HILIC column; Waters, Waltham, MA, 50x2.1mm, 2.5μm) and reversed phase liquid chromatography (RPLC; C18 column; Higgins Analytical, Mountain View, CA, 50x2.1mm, 2.6 μm).