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MB Sample ID: SA237138
Local Sample ID: | HepG2-C07 |
Subject ID: | SU002455 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Treatment:
Treatment ID: | TR002467 |
Treatment Summary: | Cells were treated by applying docosahexaenoic acid (DHA) conjugated to bovine serum albumin (BSA). DHA was obtained from Avanti Polar lipids (Alabaster, AL, USA) and dissolved in 100% ethanol at a concentration of 100 mM. 25 μL of DHA stock or 25 μl of 100% ethanol (CON) was added per 50 mL of normal growth media containing 2% (w/v) of fatty acid-free BSA. Media was sterile filtered by passing it through a 0.2 μM PES filter, and then both CON and DHA-containing media were incubated in a water bath for 2 hours at 55ºC to conjugate DHA to BSA. Conjugated media was stored at 4ºC until used. Cells were incubated overnight in the presence of pre-warmed CON or DHA-conjugated media before harvest. |