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MB Sample ID: SA242762
Local Sample ID: | Pyruvate_Soleus_13CNMR-3 |
Subject ID: | SU002515 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 16±3 weeks |
Gender: | Not applicable |
Animal Animal Supplier: | Jackson Labs (Stock # 000664) |
Animal Housing: | Housed in a temperature of 22 oC |
Animal Light Cycle: | 12-hour light/12-hour dark |
Animal Feed: | Ad libitum chow diet food |
Animal Water: | free access to food and water (3-5 animals per cage). |
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Treatment:
Treatment ID: | TR002527 |
Treatment Summary: | Mice were anesthetized (using a combination of xylazine (80mg/kg) and ketamine (10mg/kg)) to allow removal of soleus and extensor digitorum longus (EDL) muscles. Upon removal, muscles were incubated at 22 oC in Ringer/MEM solution gas equilibrated with 95/5% O2/CO2 with appropriate 13C labeled substrates in a perfusion chamber routinely used for isolated muscle mechanics for 30 minutes. These included the following: 5.5 mM [U-13C6] glucose; 5.5 mM [U-13C3] pyruvate, or 16.5 mM [13C2] labeled Na-acetate. Following incubation, muscles were quickly removed, blotted, and then rapidly frozen in liquid nitrogen for subsequent NMR analysis. N=4 muscles were pooled into a single biological replicate of 30-50 mg tissue to afford detectable levels of substrates in the NMR analysis. |
Animal Vet Treatments: | none |
Animal Anesthesia: | a combination of xylazine (80mg/kg) and ketamine (10mg/kg)) |
Animal Fasting: | non-fasted |
Animal Endp Euthanasia: | Euthanasia was carried out by thoracotomy followed by cervical dislocation. |
Animal Endp Tissue Coll List: | Skeletal muscle (soleus and EDL) |