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MB Sample ID: SA245787

Local Sample ID:bivalves_21C_C125_02
Subject ID:SU002545
Subject Type:Invertebrate
Subject Species:Ruditapes philippinarum
Taxonomy ID:129788
Height Or Height Range:length: 3.81 ± 0.42 cm; and width: 3.06 ± 0.51 cm
Gender:Male and female

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Treatment:

Treatment ID:TR002557
Treatment Summary:Sea warming and 17-α-ethinylestradiol exposure. After depuration, the organisms were subjected to a chronic toxicity test for 28 days, consisting of exposure to five different EE2 concentrations: 0 (control group), 5, 25, 125 and 625 ng/L. To assess the effects of a warming scenario on the impacts of EE2, the experiments were carried out at 17 ± 1 °C (control; mean temperature of sampling area during September) and at 21 ± 1 °C (worst-case climate change scenario). The aquaria were placed in distinct climatic rooms for each temperature. To reach 21 °C, the temperature was raised by 2 °C, every 2–3 days. For each concentration level and temperature, 12 samples were considered: 4 individuals per aquarium and 3 aquaria per treatment. In each aquarium, a total of 3 L of artificial seawater (salinity: 30 ± 1), continuous aeration, and a natural photoperiod were used. The exposure medium for each condition was renewed weekly, after which EE2 concentration levels were re-established. Mortality was checked daily and found to be null. At the end of the 28-day exposure period, the clams were frozen in liquid nitrogen and stored at – 80 ºC.
Treatment Protocol Filename:Bivalves_Experimental_Procedure.docx
Treatment Compound:Sea warming and 17-α-ethinylestradiol exposure
Treatment Dose:Clams were exposed to five different EE2 concentrations: 0 (control group), 5, 25, 125 and 625 ng/L. To assess the effects of a warming scenario on the impacts of EE2, the experiments were carried out at 17 ± 1 °C (control) and at 21 ± 1 °C (worst-case climate change scenario).
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