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MB Sample ID: SA249766
Local Sample ID: | MED1 Library 1 uM Condensate Sample 2 |
Subject ID: | SU002593 |
Subject Type: | Synthetic sample |
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Treatment:
Treatment ID: | TR002605 |
Treatment Summary: | Chemical libraries were added with individual molecules at a concentration of 100 nM, 1 uM or 10 uM to the condensate-forming low-complexity domain MED1 tagged with mCherry. Condensates were stimulated with 150 nM RNA. Condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction. |