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MB Sample ID: SA256211
Local Sample ID: | 180968_3_GREENL_ONC_OS_7dpi_POS |
Subject ID: | SU002651 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
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Treatment:
Treatment ID: | TR002663 |
Treatment Summary: | The C57BL/6 mice were injected with AAV2-CNTF at titer of 1x10e13 vg/ml (viral genomes per mL) with 1 uL of AAV-CNTF injected in each eye. Mice labeled as AAV2-Green-Lantern were injected as a control. Prior to surgery, mice received a cocktail of ketamine and xylazine for anesthesia. The optic nerve crush was performed by exposing the optic nerve by blunt dissection. The nerve was crushed using forceps for 10 seconds 1 mm behind the globe. At 7 and 14 days post crush mice were euthanized and optic nerves were collected. |