Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA256217

Local Sample ID:	180969_2_CNTF_ONC_OS_7dpi_POS
Subject ID:	SU002651
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Male and female

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Treatment:

Treatment ID:	TR002663
Treatment Summary:	The C57BL/6 mice were injected with AAV2-CNTF at titer of 1x10e13 vg/ml (viral genomes per mL) with 1 uL of AAV-CNTF injected in each eye. Mice labeled as AAV2-Green-Lantern were injected as a control. Prior to surgery, mice received a cocktail of ketamine and xylazine for anesthesia. The optic nerve crush was performed by exposing the optic nerve by blunt dissection. The nerve was crushed using forceps for 10 seconds 1 mm behind the globe. At 7 and 14 days post crush mice were euthanized and optic nerves were collected.

Treatment ID:

TR002663

Treatment Summary:

The C57BL/6 mice were injected with AAV2-CNTF at titer of 1x10e13 vg/ml (viral genomes per mL) with 1 uL of AAV-CNTF injected in each eye. Mice labeled as AAV2-Green-Lantern were injected as a control. Prior to surgery, mice received a cocktail of ketamine and xylazine for anesthesia. The optic nerve crush was performed by exposing the optic nerve by blunt dissection. The nerve was crushed using forceps for 10 seconds 1 mm behind the globe. At 7 and 14 days post crush mice were euthanized and optic nerves were collected.