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MB Sample ID: SA258368
| Local Sample ID: | PTEN WT Rep2 |
| Subject ID: | SU002674 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | embryonic fibroblasts |
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Treatment:
| Treatment ID: | TR002686 |
| Treatment Summary: | media void of cystine was supplemented with 3,3’- 13C2-cystine to 63 mg/L. On day 0, Pten WT and Pten KO MEFs were seeded at 80% confluency in standard media in 10cm plates. After 24 hours, the standard media was replaced with the heavy isotope labeled media for 12 hours before metabolites were extracted as described in the Sampleprep section. N = 5. |
