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MB Sample ID: SA288554
| Local Sample ID: | A677G_3_neg |
| Subject ID: | SU002840 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Cell Biosource Or Supplier: | American Tissue Culture Collection (ATCC) |
| Cell Strain Details: | HEK-293T |
| Cell Passage Number: | 2 |
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Treatment:
| Treatment ID: | TR002849 |
| Treatment Summary: | HEK-293T cells at 80% confluency were infected in the presence of polybrene for 16 h in DMEM with lentivirus constructs containing either wild-type (WT) EZH2, A677G-mutant (A677G) EZH2, Y641F-mutant (Y641F) EZH2, or H689A/F667I-mutant (DM) EZH2. Infected cells were selected for resistance to blasticidin (10 μg/mL) for 10 days to ensure monoclonal populations. |
| Cell Media: | DMEM supplemented with 10%FBS and 1% Pen/Strep |
| Cell Pct Confluence: | 80 |
