Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA314487

Local Sample ID:	Ala-40-2-1
Subject ID:	SU002990
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR002999
Treatment Summary:	To trace L-Alanine metabolism, RAW264.7 cell were grown in DMEM (Hyclone) supplemented with 10% (v/v) cosmic calf (Hyclone), then transferred into L-Alanine-free medium and deprived of serum overnight. Subsequently, cells were incubated with 5 mM L-Alanine and 5mM [U-13C]-L-Alanine in serum-starved medium (DMEM/0.5% serum). Additionally, fresh media containing L-Alanine and [U-13C]-L-Alanine were exchanged 2 h before metabolite extraction for metabolic analysis.

Treatment ID:

TR002999

Treatment Summary:

To trace L-Alanine metabolism, RAW264.7 cell were grown in DMEM (Hyclone) supplemented with 10% (v/v) cosmic calf (Hyclone), then transferred into L-Alanine-free medium and deprived of serum overnight. Subsequently, cells were incubated with 5 mM L-Alanine and 5mM [U-13C]-L-Alanine in serum-starved medium (DMEM/0.5% serum). Additionally, fresh media containing L-Alanine and [U-13C]-L-Alanine were exchanged 2 h before metabolite extraction for metabolic analysis.