Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA316293

Local Sample ID:	MDA03-01-4742
Subject ID:	SU003023
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment:

Treatment ID:	TR003032
Treatment Summary:	P65 plasmid tagged with GFP (Addgene plasmid #23255) was used for transfecting MDA-MB-231 cells. Transfection was performed as described previously [8]. In brief, following the seeding of MDA-MB-231 cells in 6 well plates, cells were transfected with 2.5 μg of RelA/P65 plasmid using lipofectamine 3000 prepared in Opti-MEM I media (Invitrogen Carlsbad, CA, USA). After cells incubated for 6 h, RPMI-1640 culture media was added to replace Opti-MEM I media. Cells were selected using Genticine antibiotic (G418) (InvivoGen, USA, #ant-gn-1).

Treatment ID:

TR003032

Treatment Summary:

P65 plasmid tagged with GFP (Addgene plasmid #23255) was used for transfecting MDA-MB-231 cells. Transfection was performed as described previously [8]. In brief, following the seeding of MDA-MB-231 cells in 6 well plates, cells were transfected with 2.5 μg of RelA/P65 plasmid using lipofectamine 3000 prepared in Opti-MEM I media (Invitrogen Carlsbad, CA, USA). After cells incubated for 6 h, RPMI-1640 culture media was added to replace Opti-MEM I media. Cells were selected using Genticine antibiotic (G418) (InvivoGen, USA, #ant-gn-1).