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MB Sample ID: SA316692
Local Sample ID: | 20120522-zhxl-A5-L-1 |
Subject ID: | SU003031 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Treatment:
Treatment ID: | TR003040 |
Treatment Summary: | Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample. |