Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA316732

Local Sample ID:	20120522-zhxl-A5-1
Subject ID:	SU003031
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Treatment:

Treatment ID:	TR003040
Treatment Summary:	Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Treatment ID:

TR003040

Treatment Summary:

Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.