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MB Sample ID: SA321848
| Local Sample ID: | 28_T0_4 |
| Subject ID: | SU003067 |
| Subject Type: | Plant |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
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Treatment:
| Treatment ID: | TR003076 |
| Treatment Summary: | Axenic cultures (1 L in L1 media, 35 ppt) of the diatom Thalassiosira pseudonana CCMP1335 were grown at different temperatures of 14, 20, and 28°C under 120 µmol photons m-2 s-1 (ULM-500 Light Meter, Walz) and a 16:8 h light:dark cycle (four replicates for each treatment). They were harvested in late exponential growth phase by filtration onto 2.0 µm Isopore filters (1 L) and stored at -20°C for later experimentation. Diatom cells were rinsed from filters into 10 mL sterile L1 media, sonicated to lyse cells for 20 min, and passed through a pre-combusted GF/F filter. R. pomeroyi was inoculated into 2.7 mL of the endometabolome samples (3 x 107 cells mL-1) and grown for 10 h. |
| Treatment Protocol Filename: | 3_Treatment protocol_UGA_temp_Oct2023_drawdown.docx |
