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MB Sample ID: SA322012
Local Sample ID: | 21 |
Subject ID: | SU003070 |
Subject Type: | Plant |
Subject Species: | Thalassiosira pseudonana |
Taxonomy ID: | 296543 |
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Treatment:
Treatment ID: | TR003079 |
Treatment Summary: | Six treatment combinations of a marine diatom strain Thalassiosira pseudonana CCMP1335 were prepared: treatments incubated axenically at either 14, 20, or 28 oC, and treatments co-cultured with a bacterial strain Ruegeria pomeroyi DSS-3 at the corresponding temperatures (four replicates for each). L1 media was used with NaH13CO3 as a source of bicarbonate and a salinity of 35 ppt. The diatom used for the co-cultured treatments was B12 stressed to emphasize the known co-existing system. The light cycle consisted of 16 h light (120 µmol photons m-2 s-1) and 8 h of dark. |
Treatment Protocol Filename: | 3_Treatment protocol_UGA_temp_Oct2023_main.docx |