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MB Sample ID: SA323533
| Local Sample ID: | R_C48h_EA_23b_1_2 |
| Subject ID: | SU003095 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | MDA-MB-231 cells |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | ATCC (Manassas, VA, USA); ATCC HTB-26 |
| Cell Strain Details: | Epithelial breast cancer cells; absence of estrogen and progesterone receptors, HER2 overexpression |
| Cell Passage Number: | Inferior to 10 |
| Cell Counts: | 5 M |
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Treatment:
| Treatment ID: | TR003104 |
| Treatment Summary: | Cells were treated during culture time by adding drugs stock solution to the growth culture medium. Briefly, after seeding, cells were allowed to adhere for 24 h (t = 0 h). After this, the experiment was initiated by adding stock solutions of each drug to achieve the corresponding half of maximal inhibitory effect IC50 values: 1.0 µM cDDP and 7.9 μM Pd2Spm. Phosphate-buffered saline (PBS) was used in control groups. Then, cells were incubated and collected at t = 24 h and t = 48 h, with basis on the population (25.5 ± 0.9 h and 30.6 ± 1.1 h for MDA-MB-231 and MDA-MB-231/R cells, respectively). |
| Treatment Compound: | cDDP or Pd2Spm (PBS for controls) |
| Treatment Route: | Dissolution of drugs stock solutions into the culture medium |
| Treatment Dose: | 1.0 µM cDDP and 7.9 μM Pd2Spm |
| Treatment Dosevolume: | 5.5 mL |
| Treatment Doseduration: | 24 h and 48 h |
| Treatment Vehicle: | Phosphate-buffered saline (PBS) |
| Cell Media: | Dulbecco’s Modified Eagle’s Medium—high-glucose cell growth medium (DMEM-HG) |
| Cell Envir Cond: | Humidified atmosphere of 5% CO2 at 37 ◦C |
| Cell Harvesting: | Tripsinization |
| Cell Pct Confluence: | ~ 70 to 80% |
