Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA327058

Local Sample ID:	5_O_HFD
Subject ID:	SU003125
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR003134
Treatment Summary:	Germfree male C57BL/6N mice were associated with 0.2 ml of the freshly thawed microbial mixtures via oral gavage three times within one week at the age of five weeks. From week 8 onwards, diet was changed from autoclaved standard chow to synthetic control diet (CD) for two weeks. Thereafter, the mice were randomly assigned to four different groups fed the following diets for eight weeks (the references and main differences in composition between the diets are provided in Figure S1B): CD, control diet; CD-BA, control diet supplemented with the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) (each 0.1%); HFD, lard-based high-fat diet (48% energy from fat); HFD-BA, HFD supplemented with CA and CDCA. These diets were used due to the link between Coriobacteriia/E. lenta and bile acid metabolism as well as host metabolic health.

Treatment ID:

TR003134

Treatment Summary:

Germfree male C57BL/6N mice were associated with 0.2 ml of the freshly thawed microbial mixtures via oral gavage three times within one week at the age of five weeks. From week 8 onwards, diet was changed from autoclaved standard chow to synthetic control diet (CD) for two weeks. Thereafter, the mice were randomly assigned to four different groups fed the following diets for eight weeks (the references and main differences in composition between the diets are provided in Figure S1B): CD, control diet; CD-BA, control diet supplemented with the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) (each 0.1%); HFD, lard-based high-fat diet (48% energy from fat); HFD-BA, HFD supplemented with CA and CDCA. These diets were used due to the link between Coriobacteriia/E. lenta and bile acid metabolism as well as host metabolic health.