Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA328186

Local Sample ID:	DIG014 DP2.Hour 2 plasma
Subject ID:	SU003145
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment:

Treatment ID:	TR003154
Treatment Summary:	This study was approved by the Florida State University Institutional Review Board and registered on clinicaltrials.gov (NCT04877262). Healthy, adults who were normal-weight (BMI:18.5-24.9 kg/m2) or overweight (BMI: 25-29.9 kg/m2) and 23-42 y, were recruited from the greater Tallahassee, Florida area between December 2021 and May 2022 and provided informed consent prior to participating. Potential participants were excluded if they had any disease or condition known to interfere with metabolism or normal gastrointestinal function (diabetes, cardiovascular disease, kidney disease, prior bariatric surgery, suspected or known fistulas, gastrointestinal obstruction, gastrointestinal disease, colonoscopy within 3 months of the study, alcoholism, substance abuse disorders); irregular menstrual cycle in the past 6 months; weight fluctuations greater that 5% during the previous 6 months; antibiotic use within 3 months of participation; allergies or intolerances to foods included in the controlled diet; habitual use of laxatives, stool softeners, or anti-diarrheal medications (≥ 1x/week); whole-gut transit time >72 h; or were pregnant or lactating. Participants followed an early time-restricted eating (eTRE) schedule (i.e., all meals consumed in a 6-h window between 0800 and 1400) and a control eating schedule (i.e., all meals consumed in a 12-h window between 0800 and 2000) for 9-d each in randomized order. On day 6 of each study period, participants arrived in the morning to the laboratory for a mixed-meal tolerance test (MMTT). Participants spent 6 hours in the laboratory to measure fasting (0h) and postprandial (2h and 4h) plasma metabolite concentrations in response to the MMTT. Whole blood was collected in EDTA vacutainer tubes (BD Vacutainer, Franklin Lakes, New Jersey, USA) and centrifuged at 3000 RPM for 15 minutes at 4°C before aliquots were frozen at -80°C until analysis.

Treatment ID:

TR003154

Treatment Summary:

This study was approved by the Florida State University Institutional Review Board and registered on clinicaltrials.gov (NCT04877262). Healthy, adults who were normal-weight (BMI:18.5-24.9 kg/m2) or overweight (BMI: 25-29.9 kg/m2) and 23-42 y, were recruited from the greater Tallahassee, Florida area between December 2021 and May 2022 and provided informed consent prior to participating. Potential participants were excluded if they had any disease or condition known to interfere with metabolism or normal gastrointestinal function (diabetes, cardiovascular disease, kidney disease, prior bariatric surgery, suspected or known fistulas, gastrointestinal obstruction, gastrointestinal disease, colonoscopy within 3 months of the study, alcoholism, substance abuse disorders); irregular menstrual cycle in the past 6 months; weight fluctuations greater that 5% during the previous 6 months; antibiotic use within 3 months of participation; allergies or intolerances to foods included in the controlled diet; habitual use of laxatives, stool softeners, or anti-diarrheal medications (≥ 1x/week); whole-gut transit time >72 h; or were pregnant or lactating. Participants followed an early time-restricted eating (eTRE) schedule (i.e., all meals consumed in a 6-h window between 0800 and 1400) and a control eating schedule (i.e., all meals consumed in a 12-h window between 0800 and 2000) for 9-d each in randomized order. On day 6 of each study period, participants arrived in the morning to the laboratory for a mixed-meal tolerance test (MMTT). Participants spent 6 hours in the laboratory to measure fasting (0h) and postprandial (2h and 4h) plasma metabolite concentrations in response to the MMTT. Whole blood was collected in EDTA vacutainer tubes (BD Vacutainer, Franklin Lakes, New Jersey, USA) and centrifuged at 3000 RPM for 15 minutes at 4°C before aliquots were frozen at -80°C until analysis.