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MB Sample ID: SA330839
| Local Sample ID: | WT_C |
| Subject ID: | SU003162 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Treatment:
| Treatment ID: | TR003171 |
| Treatment Summary: | Mice were group-housed and fed with standard chow. B lympchocytes from wild-type and knock-out mice were isolated and cultured for 24 hours in RPMI 1640 supplemented with 10% FCS, 1mM Glutamine, 0,01% sodium pyruvate, 20mM 2-mercaptoethanol, penicillin and streptomycin. Cells were cultured adding interleukine-4 and interleukine-5 at 10 ng/Ml, anti-IgM 1 ug/mL and anti-CD40 0,3 ug/mL. |
