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MB Sample ID: SA331979

Local Sample ID:R_cont_3
Subject ID:SU003179
Subject Type:Plant
Subject Species:Amaranthus caudatus L.
Taxonomy ID:3567
Gender:Not applicable

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Treatment:

Treatment ID:TR003188
Treatment Summary:Amaranthus caudatus L., variety Karwa dauta plants were used in the study. After two weeks culturing in the hydroponic system (nutrient solution (in mmol/L) was as follows: Ca(NO3)2·4H2O - 3.81; KNO3 - 6.44; MgSO4·7H2O - 0.81; KH2PO4 - 1.83; NH4NO3 - 0.87; Fe-EDTA - 0.09; H3BO3 - 0.047; MnSO4·5H2O - 0.007; ZnSO4·7H2O - 0.0007; CuSO4·5H2O - 0.0008; (NH4)2MoO4 - 0.0005), the vessels with six-week-old plants (experimental group) were subjected to Zn2+ stress for one week which was accomplished by supplementation of 300 µmol/L ZnSO4·7H2O in the nutrient solution. Control plants remained untreated. Roots, young and mature leaves of seven-week-old Zn-treated and control plants were collected separately.
Treatment Protocol Comments:6 sample groups: LY_cont - young leaves of control plants; LY_Zn - young leaves of Zn-treated plants; LM_cont - mature leaves of control plants; LM_Zn - mature leaves of Zn-treated plants; R_cont - roots of control plants; R_Zn - roots of Zn-treated plants.
Treatment:Heavy metal stress
Treatment Compound:ZnSO4·7H2O
Treatment Route:supplementation in the nutrient solution
Treatment Dose:300 µmol/L
Treatment Doseduration:1 week
Plant Plot Design:total 27 plants in nine vessels
Plant Light Period:16 : 8 day/night regimen
Plant Humidity:70-75% relative humidity
Plant Temp:day/night temperatures of 24/18° C
Plant Watering Regime:plant were culturing in the hydroponic system
Plant Nutritional Regime:nutrient solution in mmol/L as follows: Ca(NO3)2·4H2O - 3.81; KNO3 - 6.44; MgSO4·7H2O - 0.81; KH2PO4 - 1.83; NH4NO3 - 0.87; Fe-EDTA - 0.09; H3BO3 - 0.047; MnSO4·5H2O - 0.007; ZnSO4·7H2O - 0.0007; CuSO4·5H2O - 0.0008; (NH4)2MoO4 - 0.0005
Plant Growth Stage:vegetative stage
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