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MB Sample ID: SA332237
| Local Sample ID: | G3 |
| Subject ID: | SU003186 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | AGS and AZ-521 |
| Species Group: | AGS and AZ-521 |
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Treatment:
| Treatment ID: | TR003195 |
| Treatment Summary: | AGS and AZ-521 cells were cultured in T-75 cell culture flasks overnight to a density of approximately 4x106 cells. Cells were incubated in media containing 20 ug/mL of purified s1m1 VacA toxin and 5 mM ammonium chloride, and supplemented with 10mM Taurine. Following intoxication, the media was removed, and cells were washed with PBS. Cells were detached by incubation with trypsin for 5 minutes and collected via centrifugation at 4°C at 1,000 rpm for 4 minutes. Trypsin was removed, cells were once again washed with PBS, and the cells were then flash-frozen in liquid nitrogen and stored at -70°C. |
| Treatment Protocol Filename: | Cell_Culture_Methods.pdf |
