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MB Sample ID: SA332987
Local Sample ID: | Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322) |
Subject ID: | SU003206 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Treatment:
Treatment ID: | TR003215 |
Treatment Summary: | CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously. |