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MB Sample ID: SA346720
Local Sample ID: | Sen_shHK2_WTHK2_2 |
Subject ID: | SU003301 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Treatment:
Treatment ID: | TR003310 |
Treatment Summary: | Stable isotope tracer analysis using 13C6-glucose was conducted in three biologically independent experiments for the following experimental groups: control proliferating cells (Pro), RAS-induced senescent cells via 4-OHT with control shRNA (Sen_shctrl), senescent cells with shRNA targeting HK2 (Sen_shHK2), senescent cells with shRNA targeting HK2 and rescued with wildtype Flag-HK2 (Sen_shHK2_WTHK2), and senescent cells with shRNA targeting HK2 and rescued with mutant Flag-HK2 (Sen_shHK2_MutHK2). The experiments were performed by seeding cells at a density of 3 × 10^5 cells per 6 cm dish and incubating them with 25 mM [13C6]-glucose tracer in glucose-free medium for 30 min. |