Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA354169

Local Sample ID:	210309_MDA_Timecourse_RSL3_n3_4h_RSL3_1uM_29
Subject ID:	SU003381
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment:

Treatment ID:	TR003390
Treatment Summary:	Treatment of brest cancer cells (MDA-MB-231 cells) for the analysis of oxidized phosphatidylethanolamine (oxPE), oxidized phosphatidylcholine (oxPC), and oxidized phosphatidylinositol (oxPI): Human MDA-MB-231 breast cancer cells were treated with vehicle (DMSO) or RSL3 (1 or 10 µM) with or without ferrostatin-1 (3 µM) for 2 h, 4 h, 6 h, or 24 h or with SCs (1, 3, and 10 µM) for 2 h at 37°C and 5% CO2. Cells were harvested, washed with PBS pH 7.4, snap-frozen, and stored at -80°C.

Treatment ID:

TR003390

Treatment Summary:

Treatment of brest cancer cells (MDA-MB-231 cells) for the analysis of oxidized phosphatidylethanolamine (oxPE), oxidized phosphatidylcholine (oxPC), and oxidized phosphatidylinositol (oxPI): Human MDA-MB-231 breast cancer cells were treated with vehicle (DMSO) or RSL3 (1 or 10 µM) with or without ferrostatin-1 (3 µM) for 2 h, 4 h, 6 h, or 24 h or with SCs (1, 3, and 10 µM) for 2 h at 37°C and 5% CO2. Cells were harvested, washed with PBS pH 7.4, snap-frozen, and stored at -80°C.