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MB Sample ID: SA355226
Local Sample ID: | gln_gluc_3_8hr |
Subject ID: | SU003402 |
Subject Type: | Cultured cells |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
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Treatment:
Treatment ID: | TR003411 |
Treatment Summary: | Bulk bovine aortic endothelial cells were cultured in complete media. These bulk plates were used to seed 96-well plates at 40,000 cells per well in 200µL of media. These cultures were maintained for 24 hours in complete media supplemented with 10% FBS before nutrient deprivation. Triplicate controls were included at every time point and were media swapped to complete media + 10% dialyzed FBS. Triplicate 96-well plates were used for nutrient deprivation experiments, and a separate plate was cultured for cell counting using a hemocytometer following manufacturer instructions. Tags were allocated to different wells to reduce bias caused by tag interactions. A single row (B) was left empty during culture. These wells were used to create a calibration curve for each analyte centered around the approximate biological concentration in controls. |