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MB Sample ID: SA358649
| Local Sample ID: | sgLuc -Gln 60 2 |
| Subject ID: | SU003427 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Cell Strain Details: | MCF7 |
| Cell Primary Immortalized: | Immortalized |
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Treatment:
| Treatment ID: | TR003436 |
| Treatment Summary: | Cells are either control (sgLuc) or ASCT2-KO (sgSLC1A5). All groups were plated in triplicate in a six well plate 48hours prior to treatment. Cells were treated with either complete media, or media containing low serine for six hours, or media lacking glutamine (-Q) for one hour prior to harvesting. |
| Treatment Doseduration: | 1-6 hours |
| Cell Growth Container: | Corning 6-well plates |
| Cell Media: | RPMI w/o Glucose, Sodium Pyruvate, Amino acids |
| Cell Pct Confluence: | 80% |
