Summary of Study ST000260
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000211. The data can be accessed directly via it's Project DOI: 10.21228/M84598 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000260 |
Study Title | Analysis of DJ-1 Knockout Mouse Brains |
Study Type | Mouse genotype comparison |
Study Summary | We analyzed metabolites in the brains of wild type mice and DJ-1 knockout mice. The DJ-1 knockout mouse is a model of an inherited form of Parkinson's disease. |
Institute | National Institute on Aging |
Department | Laboratory of Neurogenetics |
Laboratory | Cell Biology and Gene Expression Section |
Last Name | Hauser |
First Name | David |
Address | 35 Lincoln Drive, BLDG 35, Room 1A-1012, Bethesda, MD 20892 |
hauserd@mail.nih.gov | |
Phone | 301-435-8995 |
Submit Date | 2015-09-17 |
Num Groups | 2 |
Total Subjects | 25 |
Raw Data Available | No |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR000294 |
Treatment Summary: | The animals were either wild type of homozygous DJ-1 knockout animals. At 4 months of age, the mice were euthanized and their brains were processed for metabolomic analysis. |
Animal Vet Treatments: | None |
Animal Fasting: | No dietary interventions |
Animal Endp Euthanasia: | The mice were euthanized with CO2 followed by cervical dislocation. |
Animal Endp Tissue Coll List: | Cerebral Hemispheres |
Animal Endp Tissue Proc Method: | Immediately after euthanization, the brains were removed and dissected on an ice cold stage. The cerebellum was removed and the two cerebral hemispheres were bisected along the longtiduinal fissue. The cerebral hemispheres were immediately frozen on dry ice in individual tubes. The frozen tissue was then sent to Metabolon for subsequent processing. |