Summary of Study ST001245
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000832. The data can be accessed directly via it's Project DOI: 10.21228/M84Q24 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001245 |
Study Title | Luteal lipids regulate progesterone production and may modulate immune cell function during the estrous cycle and pregnancy |
Study Summary | Despite data indicating an important functional role for bioactive lipids in luteal function, little is known about the patterns of abundance of these lipids in corpus luteum (CL) during luteal development, maintenance, and rescue, in any species. Therefore, the abundance of lipid mediators, including endocannabinoids and oxylipins from cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP)-dependent metabolism were profiled in the CL on days 4, 11, and 18 of the estrous cycle and on day 18 of pregnancy. The objectives of this study were to identify lipid mediators that regulate luteal function during these transitions, to integrate the lipid profile with a previously published mRNA profile of CL during maternal recognition of pregnancy, and to determine the effect of a subset of lipids on in vitro progesterone production. |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Submit Date | 2019-07-29 |
Publications | Hughes, C.H.K., R. Bosviel, J.W. Newman, J.L. Pate. Luteal lipids regulate progesterone production and may modulate immune cell function during the estrous cycle and pregnancy. Front. Endocrinol. Jun 27, 2019 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2019-09-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR001328 |
Treatment Summary: | For cows assigned to the day 4 group, upon observation of estrus and a dominant follicle by ultrasound, cows were given an injection of GnRH (Factrel, 100 µg; Zoetis) in order to precisely time ovulation relative to time of collection for these early CL. Cows were slaughtered on day 4 following estrus. For samples collected later than day 4, precise synchrony of ovulation relative to CL collection was not necessary, so no GnRH was given, and CL were collected via colpotomy. For CL of pregnancy, cows were bred by artificial insemination and a uterine flush was performed immediately following CL collection and was examined for embryo fragments to confirm the presence of a viable pregnancy. For all samples, tissue was snap frozen in liquid nitrogen immediately following tissue collection and stored at -80 degrees Celsius thereafter. For in vitro experiments, three to five dairy cows were used in each group, CL were collected on day 10-12 of the estrous cycle, and each treatment was applied to cells from each cow |