Summary of Study ST001421
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000975. The data can be accessed directly via it's Project DOI: 10.21228/M8NX1G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001421 |
Study Title | Metabolomic study of Escherichia coli K-12 MG1655 WT and its transcriptional regulator mutants under anaerobic fermentation conditions |
Study Type | Metabolomics |
Study Summary | Metabolomic analysis of Wildtype, fnr, arcA and ihf mutants in glucose minimal media under anaerobic fermentation conditions during its exponential phase of growth. Three biological and two technical replicate samples (n=6) were harvested for each of the strains while growing in a bioreactor anaerobically at 37 degrees Celsius and 150 rpm. This study aims to characterize and compare the metabolic profiles of all these strains. |
Institute | IIT Bombay |
Department | Department of Chemical Engineering |
Laboratory | Systems Biology and Metabolic Engineering Laboratory (SBMEL) |
Last Name | Pal |
First Name | Ankita |
Address | Department of Chemical Engineering, IIT Bombay, Mumbai, Maharashtra, 400076, India |
pal.ankita90@gmail.com | |
Phone | 08879536816 |
Submit Date | 2020-07-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-01 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001510 |
Treatment Summary: | The WT, fnr, arcA and ihf mutants were grown in a bioreactor in glucose minimal media under anaerobic fermentation conditions at 37 degrees Celsius and 150 rpm. The samples were harvested during its exponential phase of growth for three biological and two technical replicates (n=6). The metabolites were extracted using the Methanol-Chloroform-Water method. |
Cell Growth Container: | Bioreactor |
Cell Media: | M9 + Glucose |
Cell Envir Cond: | 37 degrees Celsius, 150 rpm. anaerobic fermentation |