Summary of Study ST001981
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001257. The data can be accessed directly via it's Project DOI: 10.21228/M87986 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001981 |
Study Title | Non-destructive characterization of Mesenchymal stem cells |
Study Summary | Culture media from the growth of three different MSC cell lines (two bone marrow, one iPSC) were sampled daily for NMR metabolomics analysis. T cell proliferation and IDO assays were used as surrogates of anti-inflammatory function. Linear regression was used to assess the media metabolic changes over time, and partial least squares regression (PLSR) was then used to obtain predictive media markers (PMMs) based on variable importance in projection (VIP) scores. In addition, pathway analysis was performed to show the relations between media metabolites (MMs) and cell metabolites (CMs). |
Institute | University of Georgia |
Last Name | Shen |
First Name | Xunan |
Address | 315 riverbend road |
xs41379@uga.edu | |
Phone | 7858407009 |
Submit Date | 2021-09-16 |
Raw Data Available | Yes |
Analysis Type Detail | NMR |
Release Date | 2021-12-01 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002074 |
Treatment Summary: | Each cell line has 10 replicates. Two bone marrow-derived MSC lines (RoosterBio, Frederick MD) lot #0071 (F, 18-30) and #0182 (F, 26) (which the manufacturer has both research and clinical-grade lots available), and one induced pluripotent stem cell derived MSC cell-line (Cellular Dynamics International, Madison WI) (Lot #0003, also prequalified) were used and referred here as BM71, BM182, and iMSC, respectively. iMSC were cultured for 25 days, BM71 were cultured for 21 days and BM182 were cultured for 23 days. 100 µl media was sampled daily from each sample and stored at -80◦C for further analysis. |