Summary of Study ST002061
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001305. The data can be accessed directly via it's Project DOI: 10.21228/M81H6N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002061 |
Study Title | Glutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine |
Study Type | Glutamine flux |
Study Summary | 107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs. |
Institute | Shanghai Jiao Tong University affiliated Renji Hospital |
Department | Department of Urology |
Laboratory | Cheng Jinke's Lab |
Last Name | Zhou |
First Name | Wei |
Address | N0.280 South Chongqing Road |
joesphchou@alumni.sjtu.edu.cn | |
Phone | +8615216716293 |
Submit Date | 2022-01-19 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-07 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002155 |
Treatment Summary: | Method was described in previous studies (Jha et al., 2015; Lauterbach et al., 2019). 107 BMDMs per group were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs. |