Summary of Study ST002201
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001404. The data can be accessed directly via it's Project DOI: 10.21228/M87M6J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002201 |
Study Title | Multi-omics profiling of 5 Arabidopsis accessions in response to combined water and nitrogen deficiencies. |
Study Type | Multi-omics analysis |
Study Summary | In this study, we build an in-depth multi-omics profile of 5 Arabidopsis accessions, in order to find the inner relations of N deficit, mild drought, and combined stress response at different scales. We used our own phenotyping platform (Phenoscope) to handle hundreds of Arabidopsis plants across 20 modalities (5 Genotypes x 2 Treatments of Water availability x 2 Treatments of Nitrogen availablity), phenotype those plants for growth-related traits and ultimately harvest the samples used in transcriptomics, metabolomics and physiological profiling. Then we have analysed those data to reveal in particular the specificities of the response to stress in each accession, as well as the W x N interactions leading to patterns that are specific to the combined stress response. The metabolomics data is particularly useful to highlight for instance the very specific profile of the Cvi-0 accession overall, or the larger extent of the metabolite responsiveness to N-deficiency with respect to mild drought. The share of the stress response that is specific to the combined stress condition is also quite variable between the 5 accessions. |
Institute | INRAE |
Department | Institut Jean-Pierre Bourgin, IJPB UMR1318 INRAE-AgroParisTech |
Laboratory | Plateforme de Chimie |
Last Name | CLEMENT |
First Name | Gilles |
Address | Etoile de Choisy (route de St Cyr) 78026 Versailles Cedex |
gilles.clement@inrae.fr | |
Phone | +33 (0) 1 30 83 31 67 |
Submit Date | 2022-06-16 |
Num Groups | 20 |
Total Subjects | 163 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2024-06-06 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002299 |
Treatment Summary: | We applied a combination of soil water content (SWC; adjusted with respect to complete soil saturation) and nutrient solution (adjusted to provide a specific amount of nitrate over the course of the experiment : 160.45 ± 7.96 ml of 5mM [Nitrate] nutrient solution in total for the control condition W+N+; 50.85 ± 4.67 ml of 5mM [Nitrate] in total for the single drought stress condition W-N+; 161.93 ± 11.07 ml of 0.5mM [Nitrate] in total for the single N-deficiency condition W+N-; 50.70 ± 4.10 ml of 1.5mM [Nitrate] in total for the combined stress condition W-N-) to establish the different conditions |