Summary of Study ST002465
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001468. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT5R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002465 |
Study Title | Map of microbially induced metabolic changes across diverse body sites in mice - Mouse Data |
Study Summary | Tissue samples from contents along the intestinal tract and systemic sites in mice that did not have any bacteria (germ free) or colonized with a simplified microbiota or more complex microbiota. |
Institute | University of Calgary |
Department | Physiology and Pharmacology |
Laboratory | McCoy |
Last Name | Brown |
First Name | Kirsty |
Address | 3330 Hospital Dr NW |
kirsty.brown12@gmail.com; kathy.mccoy@ucalgary.ca | |
Phone | 2508692232 |
Submit Date | 2023-02-04 |
Total Subjects | 72 |
Num Males | 36 |
Num Females | 36 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2023-02-05 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002567 |
Treatment Summary: | GF and OMM12-colonized gnotobiotic mice were housed in flexible-film isolators within the International Microbiome Centre (IMC) and SPF mice were maintained in the Mouse Barrier Unit (MBU) at the University of Calgary. GF mice were routinely tested for the absence of bacteria by aerobic and anaerobic culture, gram staining, and vital dye (DNA-dye Sytox green) staining of caecal contents and all mice were routinely screened for the presence of pathogens. For SPF colonization by co-housing, 8-week-old GF animals were exported, transferred into sterile individually ventilated cages and transported from the IMC to the MBU where a colonizer SPF mouse was transferred into each cage. For OMM12 colonization by co-housing, 8-week-old GF animals were transferred into isolators containing OMM12-colonized mice and an OMM12-colonized mouse was added to each cage. All animals were fed identical autoclaved diets and maintained with 12-hour light-dark cycle. |