Summary of Study ST002743
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001708. The data can be accessed directly via it's Project DOI: 10.21228/M8XM8H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002743 |
Study Title | Metabolomics comparison of lung fibroblasts from Pteropus alecto and Homo sapiens |
Study Type | MS Quantitative analysis |
Study Summary | Targeted Metabolomics was performed to identify differences in central carbon metabolism between lung fibroblasts from black flying fox fruit bat Pteropus alecto (PaLung cells) and Humans (WI-38 cells). For both PaLung and WI-38 cells, metabolomics measurements were carried out for n = 3 biological replicates. |
Institute | Duke-NUS Medical School |
Last Name | Jagannathan |
First Name | Narendra Suhas |
Address | 8 College Road, Singapore, Singapore, 169857, Singapore |
gmsnsja@nus.edu.sg | |
Phone | (65) 66015073 |
Submit Date | 2023-06-25 |
Analysis Type Detail | LC-MS |
Release Date | 2024-06-27 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002859 |
Treatment Summary: | PaLung and WI-38 cells were washed with 5% (w/w) mannitol twice and fixed with methanol containing the internal standard solution. The internal standard solution was provided by HMT. Lysates were centrifuged at 2,300 x g for 5 min at 4°C, and supernatants were filtered using the provided filter units by centrifuging at 9,100 × g for 3 hours at 4 °C. Filtrates were evaporated by SpeedVac Concentrator SPD1010 (Thermo Fisher Scientific) and submitted to HMT for analysis. The total cell numbers at the time of harvest were about 1.4 x 107 cells for each cell line. Targeted quantitative analysis was performed by HMT using capillary electrophoresis mass spectrometry (CE-TOFMS and CE-QqQMS). Absolute abundances (normalized by cell numbers) were obtained for a total of 116 metabolites (54 and 62 metabolites in the cation and anion modes, respectively). |