Summary of Study ST003282
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002034. The data can be accessed directly via it's Project DOI: 10.21228/M8PV5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003282 |
Study Title | 96-plex metabolomics studies on nutrient-deprived endothelial cells |
Study Summary | A NeuCode tag which allows for cost efficient incorporation of isotopes is used to enable a 96-plex assay for small molecule metabolomics. A 96-well plate of cell lysates is analyzed simultaneously, providing absolute quantitation of 38 metabolites across 96 samples in a single LC-MS injection. |
Institute | Saint Louis University |
Last Name | Armbruster |
First Name | Michael |
Address | 4448 Swiss Stone Ln E Apt 3C, Ypsilanti, Michigan, 48197, USA |
armbrustermr@gmail.com | |
Phone | 3145501620 |
Submit Date | 2024-02-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-07-15 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR003411 |
Treatment Summary: | Bulk bovine aortic endothelial cells were cultured in complete media. These bulk plates were used to seed 96-well plates at 40,000 cells per well in 200µL of media. These cultures were maintained for 24 hours in complete media supplemented with 10% FBS before nutrient deprivation. Triplicate controls were included at every time point and were media swapped to complete media + 10% dialyzed FBS. Triplicate 96-well plates were used for nutrient deprivation experiments, and a separate plate was cultured for cell counting using a hemocytometer following manufacturer instructions. Tags were allocated to different wells to reduce bias caused by tag interactions. A single row (B) was left empty during culture. These wells were used to create a calibration curve for each analyte centered around the approximate biological concentration in controls. |